MOESM8 of Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Taisuke Seike, Yosuke Kobayashi, Takehiko Sahara, Satoru Ohgiya, Yoichi Kamagata, Kazuhiro Fujimori
2019 Figshare  
Additional file 8: Figure S3. Micro-aerobic fermentation of strains expressing mutated XIs on medium containing d-glucose and d-xylose. Batch fermentation assays were performed under the same conditions as for Fig. 2. The fermentation properties were measured for seven strains without improved d-xylose consumption rates: SS84 (LpXI-K136T/A176T), SS85 (LpXI-Y13H/D228V), SS86 (LpXI-T273A), SS87 (LpXI-D207G), SS88 (LpXI-N223I), SS91 (LpXI-E114G) and SS94 (LpXI-L304S). These mutated LpXI expression
more » ... cassettes were introduced into the AUR1 locus of the SS29 parental strain chromosome. The dots in the panels represent the concentrations of the following metabolites in a single experiment performed in biological triplicate: glucose (blue), xylose (red), xylitol (yellow green), glycerol (purple), acetate (light blue) and ethanol (orange). The details of metabolite concentrations are shown in Additional file 9: Table S6.
doi:10.6084/m9.figshare.8240162 fatcat:djnx24jt2vgizjfj5jako5vi3y