Isolation and Identification of β-sitosterol, 7-hydroxystigmast-22-en-3,6-dione and 3β, 24(S)-dihydroxycholesta-5, 25-diene-7-one from stem bark of Nauclea pobeguinii

Adewusi John Adepoju, Sunday Olusegun Oladoye, Ezekiel Temidayo Ayodele, Adeola Victoria Falade, Gin Keat Lim, Chuan Wei OO
2022 Majalah Obat Tradisional  
The stem bark of the Nauclea pobeguinii was collected, air-dried, and pulverized and was extracted with solvent of varying polarity (n-hexane, ethyl acetate, and ethanol) to obtain the crude extracts. Silica gel column and thin layer chromatographic separation afforded three compounds whose structures were elucidated as β-sitosterol (1), 7-hydroxystigmast-22-en-3.6-dione (2), and 3β, 24(S)-dihydroxycholesta-5, 25-dien-7-one (3) by analysis of their chemical and spectral characteristic from 1D
more » ... d 2DNMR, FTIR and by comparing of data with those reported in the literature.
doi:10.22146/mot.77324 fatcat:f3dsphbc65d3xm5a4cazhuywoe