DETECTION AND IDENTIFICATION OF BEET MOSAIC POTY VIRUS (BTMV) INFECTING SUGAR BEET PLANTS IN EGYPT

R Omar, S Sidaros, S El-Kewey, Hayam Abd El-Kader, A Deif, Jehan Abass
2006 J. Biotechnol   unpublished
The virus was isolated from naturally infected sugar beet plants and identified as BtMV according to symptomatology, host range, and modes of transmission, electron microscopy and immunological and molecular detection. Host range studies revealed that, BtMV infected 28 plant species belonging to 6 families. BtMV was not easily sap transmitted, not transmitted through seeds but was readily transmitted by Myzus persicae Sulz. and Aphis fabae Scorp. The electron microscope preparations revealed
more » ... xuous filamentous virus particles 715-775 nm in length. The purified virion protein of BtMV and the total protein extracted from infected beet tissues showed a strong cross reaction with Bean Yellow Mosaic virus (BYMV) polyclonal antibodies in dot blot immunoassay indicating a higher degree of relatedness. First strand cDNA synthesis of BtMV was synthesized by priming the 3 terminal poly (A) tail of the viral RNA with Oligo (dT). For cDNA amplification, Potyvirus specific primers corresponding to the non-coding region upstream of the coat protein gene was used. A PCR fragment of the expected size approximately 220 bp was amplified and its size was estimated by agarose gel electrophoresis.
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