Storage and plant regeneration from encapsulated shoot tips of Rauvolfia serpentina — An effective way of conservation and mass propagation

A. Ray, S. Bhattacharya
2008 South African Journal of Botany  
Encapsulated shoot tips of Rauvolfia serpentina were stored at 3 different temperatures (20°C, 12°C and 4°C). Synseeds converted randomly after 4 weeks of storage at 12°C and 20°C. However, they demonstrated the most promising result at 4°C where storage achieved up to 14 weeks with high percentage of regrowth throughout the period (68.5-100%). Extending the storage period beyond 14 weeks resulted in drastic loss of viability of the explants. The synseed-derived plantlets after root induction
more » ... er root induction hardened, acclimatized and survived with 80% success. This study elucidates an effective in vitro conservation technique that can also be implemented for easy propagation and exchange of the plant species.
doi:10.1016/j.sajb.2008.06.002 fatcat:itnshc6vhjd5xm4s5zsgnvlr2y