Peer Review #4 of "Silk derived formulations for accelerated wound healing in diabetic mice (v0.2)" [peer_review]

2021 unpublished
The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods: Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by
more » ... aluated by determining the percent wound contraction, healing time and histological analysis. Results: The results indicated that the best biocompatible silk combination was of 5 % silk fibroin and 5 % Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5±0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion: It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds. PeerJ reviewing PDF | (ABSTRACT 12 Background: The present study aimed to prepare effective silk derived formulations in 13 combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic 14 mice. Methods: Diabetes was induced in albino mice by using alloxan monohydrate. After 15 successful induction of diabetes in mice, excision wounds were created via biopsy puncture 16 (6mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in 17 combination with 5% Aloe vera gel was evaluated by determining the percent wound 18 contraction, healing time and histological analysis. Results: The results indicated that the best 19 biocompatible silk combination was of 5 % silk fibroin and 5 % Aloe vera gel in which wounds 20 were healed in 13 days with wound contraction: 98.33± 0.80%. In contrast, the wound of the 21 control group (polyfax) healed in 19 day shaving 98.5±0.67% contraction. Histological analysis 22 revealed that the wounds which were treated with silk formulations exhibited an increased 23 growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion: It can be 24 concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that PeerJ reviewing PDF | (Manuscript to be reviewed 25 can be utilized in wound dressings and to prepare more innovative silk based formulations for 26 speedy recovery of chronic wounds. 27
doi:10.7287/peerj.10232v0.2/reviews/4 fatcat:ch2fqtlmi5do5j2yzvurgxu4cm