Respiratory disease in ball pythons ( Python regius ) experimentally infected with ball python nidovirus

Laura L. Hoon-Hanks, Marylee L. Layton, Robert J. Ossiboff, John S.L. Parker, Edward J. Dubovi, Mark D. Stenglein
2018 Virology  
A B S T R A C T Circumstantial evidence has linked a new group of nidoviruses with respiratory disease in pythons, lizards, and cattle. We conducted experimental infections in ball pythons (Python regius) to test the hypothesis that ball python nidovirus (BPNV) infection results in respiratory disease. Three ball pythons were inoculated orally and intratracheally with cell culture isolated BPNV and two were sham inoculated. Antemortem choanal, oroesophageal, and cloacal swabs and postmortem
more » ... and postmortem tissues of infected snakes were positive for viral RNA, protein, and infectious virus by qRT-PCR, immunohistochemistry, western blot and virus isolation. Clinical signs included oral mucosal reddening, abundant mucus secretions, open-mouthed breathing, and anorexia. Histologic lesions included chronic-active mucinous rhinitis, stomatitis, tracheitis, esophagitis and proliferative interstitial pneumonia. Control snakes remained negative and free of clinical signs throughout the experiment. Our findings establish a causal relationship between nidovirus infection and respiratory disease in ball pythons and shed light on disease progression and transmission. (M.D. Stenglein). Virology xxx (xxxx) xxx-xxx 0042-6822/
doi:10.1016/j.virol.2017.12.008 pmid:29329683 fatcat:pg7my652zzhnrhtjp4y6lpjugy