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A highly sensitive and simplified method for the luminometric determination of plasma metabolites has been developed. Furthermore, the technique has been automated for the Dynatech ML2250 Microtiter Plate Lurninometer and can be applied to the measurement of any plasma metabolite which may be coupled to a reaction involving the reduction of NAD + . Assays are described for lactose/galactose, -hydroxybutyrate and £>-lactate, and have been validated with plasma samples. The assays require 1 -2 μΐdoi:10.18452/13299 fatcat:wwwjql3vqnecpig42kxetlkwlu