Internet Archive Scholar

Variations of Histone Acetyltransferase 300 in Patients with Human Papillomavirus Type 6-Associated Anogenital Warts

Mana zakeri, Alireza Mohebbi, Fatemeh Sana Askari, Mohammad Yasaghi, Department of biology, Islamic Azad University, Tehran Medical Branch, Tehran, Iran, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranStem Cell Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran, Department of Biology, Islamic Azad University of Tehran, Medical Branch, Tehran, Iran
2020 Medical Laboratory Journal  

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (592.6 kB)
https://web.archive.org/web/20210817102202/https://mlj.goums.ac.ir//files/site1/user_files_c5015c/manazakeri-A-10-873-3-f921510.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL. The file type is application/pdf.

and objectives: Pathogenesis of human papillomaviruses (HPVs) is controlled by viral and host factors, among which human histone acetyltransferase p300 (EP300) plays an important role. This study aimed to examine single nucleotide polymorphisms (SNPs) at the EP300 binding site in patients with HPV-associated anogenital wart. Methods: After DNA extraction, polymerase chain reaction was performed to determine HPV genotypes. Human p300 was amplified to detect SNPs using Sanger sequencing. Results:
more » ... Overall, 35.3% of HPV-6-positive patients had Ile997Val substitution at the EP300 binding site. Another SNP containing A to G point mutation leading to Glu983Gly was also detected. In addition, Ile997Val substitution of EP300 was frequently observed in the patients. Conclusion: Our findings suggest that the EP300 genotype Ile/Val can be involved in HPV-6 pathogenesis. In addition, we introduced a new genotype (Glu983Gly) at the EP300 bromodomain site, which requires further investigation.
doi:10.52547/mlj.14.6.10 fatcat:aogzn6ulbzgl3nqf6qeczhj4xu
DOAJ
Citation

Mana zakeri, Alireza Mohebbi, Fatemeh Sana Askari, Mohammad Yasaghi, Department of biology, Islamic Azad University, Tehran Medical Branch, Tehran, Iran, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranStem Cell Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran, Department of Biology, Islamic Azad University of Tehran, Medical Branch, Tehran, Iran. "Variations of Histone Acetyltransferase 300 in Patients with Human Papillomavirus Type 6-Associated Anogenital Warts." Medical Laboratory Journal 14.6 (2020) 10-16