Two methods for extraction of high-purity genomic DNA from mucoid Gram-negative bacteria
English

Xiao Xiao, Zhang Jingling, Zhang Quanyi, Wang Li, Tan Yafang, Guo Zhaobiao, Yang Ruifu, Qiu Jingfu, Zhou Dongsheng
2011 African Journal of Microbiology Research  
Due to the production of abundant capsular polysaccharides and or exopolysaccharides, some bacteria form mucoid and opaque colonies on solid agar medium. Polysaccharides interfere with the isolation and characterization of DNA. Extraction of polysaccharide-free DNA from the mucoid bacteria is required for genetic and biochemical studies. In this study, mucoid Vibrio parahaemolyticus and Klebsiella pneumonia were used as model organisms for comparing the effectiveness of two DNA extraction
more » ... s. The two methods were proved reproducible and cost-effective for extracting highpurity genome DNA from mucoid bacteria for further applications.
doi:10.5897/ajmr11.785 fatcat:hztg2dwy75bbngibwb423dui6y