Approach to an Organo-Typical Environment for Cultured Cells and Tissues

W.W. Minuth, S. Kloth, J. Aigner, M. Sittinger, W. Röckl
1996 BioTechniques  
If cells or tissues are taken out of an organ and put in culture, normally they lose morphological, physiological and biochem ical features. This dedifferentiation process starts during the isolation procedure and continues during the whole culture period . It is caused by the stagnant liquid condition and the inadequate anchorage ofcells at the bottom oftissue culture pla sticware. The use offilters as basement membrane substitutes and the coating of cultureware with extracellular matrix
more » ... llular matrix proteins improve the environmental factors for cultured cells but do not consider the para crine influence ofcytokines or the nutritional needs of individual cell types. To limit cellular dedifferentiation in culture, we constructed a new system , which adapts , as far as possible, cell and tissue cultures to an organo-typical environment. The system is based on a compatible cell carrier arrangement, which allows individual selection of supports for optimal cell anchorage and differentiation. The cell carriers are placed in a newly constructed container, which is perman ently perfused with fresh culture medium. The system runs outside an incubator with simple laboratory tools; only a peristaltic pump, a warming table and pii-stabilized media are necessary. Without any subculturing, acute and chronic influences ofdrugs or the quality of medical implantation grafts can be studied over month s. Address cor respondence to Will W. Minuth ,
doi:10.2144/19962003498 pmid:8679212 fatcat:4kegb22c4zhhbjiv24jh32db2a