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If cells or tissues are taken out of an organ and put in culture, normally they lose morphological, physiological and biochem ical features. This dedifferentiation process starts during the isolation procedure and continues during the whole culture period . It is caused by the stagnant liquid condition and the inadequate anchorage ofcells at the bottom oftissue culture pla sticware. The use offilters as basement membrane substitutes and the coating of cultureware with extracellular matrixdoi:10.2144/19962003498 pmid:8679212 fatcat:4kegb22c4zhhbjiv24jh32db2a