Complementation of the rev gene mutation among human and simian lentiviruses

H Sakai, R Shibata, T Miura, M Hayami, K Ogawa, T Kiyomasu, A Ishimoto, A Adachi
1990 Journal of Virology  
The functional exchangeability of the rev gene was assessed in transient transfection experiments by using in vitro-constructed rev and gag mutants of the following three primate lentiviruses: human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus from the African green monkey (SIVAGM). Cotransfection into SW480 cells of the rev and gag mutants derived from the DNA of each infectious virus resulted in the generation of progeny particles as determined by reverse
more » ... mined by reverse transcriptase assay. rev gene mutants of HIV-2 and SIVAGM were also complemented by all gag mutants derived from the three viruses. In contrast, no evidence of complementation was obtained following cotransfection of the HIV-1 rev mutant and the gag mutant of HIV-2 or SIVAGM.
doi:10.1128/jvi.64.5.2202-2207.1990 fatcat:pfxngqgn75fkfp2q6hfwo7zmx4