THE ROLE OF ENOSGENE POLYMORPHISM IN THE RISK OF ASTHENOZOOSPERMIA INCIDENCE IN A SAMPLE OF IRAQI PATIENTS

Istikrar M. Hade, Ismail A. Abdul-Hassan
2020 Annals of Tropical Medicine and Public Health  
Single nucleotide polymorphisms are considered as one of causes of male infertility. The eNOS is primarily responsible that create of NO in the vascular endothelium, therefore eNOS is mainly expressed in thehuman testicular endothelial cells, in addition of Sertoli cells and Leydig, epididymis and vas deferens which regulates the synthesis of NO. Many study informed in the first time about the NO that is synthesized by human male gamete. Experimental evidence has shown that NO in high
more » ... O in high concentrations can cause defective function of the sperm and low concentrations of NO play very important rolein the control of the sperm physiology. In this study, the relationship of eNOS polymorphism (rs1799983 T>A)in exon 8, was studied in 50 asthenozoospermic patients and 50 fertile men. Analysis of these SNPs was performed using real time thermocycler(Real-time PCR). As related with eNOS genes rs1799983T>A SNP, TT (homozygous), no significant differences in frequency percentage were noted between fertile men subjects and asthenozoospermia patients Whereas, the frequency of heterozygous TA genotype in asthenozoospermia patients are higher than fertile men group, but showed no risk factor.As related withAA (homozygous) showed a risk factor to cause infertility.
doi:10.36295/asro.2020.2346 fatcat:ymjreaffrvhp3oi3tusr72imge