13C NMR analysis of Antarctic cryptogam extracts

B. E. Chapman, D. J. Roser, R. D. Seppelt
1994 Antarctic Science  
Water solublecompoundswere extractedfrom the dominant cryptogams of the Windmill Islands, Wilkes Land, and compared with standard polyols, sugars and amino acids using 13C nuclear magnetic resonance (NMR) spectroscopy. Previous findings for sugars and polyols from gas liquid chromatography were validated and extended. Arabitol, ribitol and mannitol were confirmed as themajor soluble carbohydrate compounds in all lichen species examined. Sucrose, fructose and glucose, but no polyols were
more » ... in two species of moss. Sorbitol was confirmed as a major component of the algae Prasiola crispa and Schizogonium murale. Mesotaenium bergrenii was confirmed to contain sucrose and glucose. No significant quantities of sugars or polyols or any other compoundwerefoundinextracts oftheredsnowalgaChloromonassp. 1. Amino acidswere detectedinthemajority of cryptogam samples and were particularly abundant in the algae P. crispa and S. murale. In the latter species the totalidentified acidsrangedfrom 13.5-66mgg-'dryweight. In additionto thecommon aminoacidcomponents of proteins, betaine and y-amino-butyric acid were detected, the latter being particularly abundant, being found widely in the moss, lichen and algae. Several unknown carbohydrates were characterized. Usnea sphacelata, U. antarctica and Pseudephebe minuscula contained a deoxy-hexitol, Grimmia antarctici contained resonance peaks consistent with a trisaccharide containing a sucrose moiety and Umbilicaria decussata possibly contained a glucose-arabitol dimer. Y N M R w a s confirmed as a powerful tool for the characterization of low molecularweight constituents of Antarctic cryptogams.
doi:10.1017/s0954102094000465 fatcat:pjpjbvazr5awldekend7t3jgnm