Methods for extraction of microorganism DNA from glacier surface snow

Yan Pei-Ying, Hou Shu-Gui, Chen Tuo, Zhang Shu-Hong, Sun Wei-Jun
2012 Sciences in Cold and Arid Regions  
In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally
more » ... e kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard-Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.
doi:10.3724/sp.j.1226.2012.00484 fatcat:vsrpybnwpvep3fwpm7aivsg6qe