Immunological and Chemical Identification of Intracellular Forms of Adenovirus Type 2 Terminal Protein

Maurice Green, Janey Symington, Karl H. Brackmann, Maria A. Cartas, Helen Thornton, Leann Young
1981 Journal of Virology  
Highly purified adenovirus type 2 terminal protein (TP) with an apparent Mr of 55,000 (55K) was prepared in quantities of 10 to 30 Ag from guanidine hydrochlorideor sodium dodecyl sulfate-disrupted virions (60 to 120 mg). Guinea pigs were immunized with 14 to 20 injections of TP in amounts of 1 to 2 ,ug. Antiserum to TP was used to study the intracellular polypeptides related to adenovirus type 2 TP. By immunoprecipitation with anti-TP serum, we identified 80K and 76K polypeptides in the
more » ... lasmic and cytoplasmic S100 fractions of [35S]methionine-labeled cells early and late after infection with Ad2. By immunoautoradiographic analysis which eliminates coprecipitation of unrelated proteins, we identified an 80K polypeptide (probably an 80K-76K doublet) in unlabeled, late infected cells, using anti-TP serum and '251-labeled staphylococcal 541 on May 9, 2020 by guest
doi:10.1128/jvi.40.2.541-550.1981 fatcat:cgxlzowtgjfjlp2eaijuz6yixe