Comparison of Several Methods for Preserving Bacteriophages

William A. Clark
1962 Applied microbiology  
CLARK, WILLIAM A. (American Type Culture Collection, Washington, D. C.). Comparison of several methods for preserving bacteriophages. Appl. Microbiol. 10:466-471. 1962-A wide variety of bacteriophages were processed and stored under different conditions to compare methods for long-term preservation. Specimens were stored for 2 years at room temperature (24 to 28 C) and at 4 C as broth lysates in 50 % glycerol, dried, and freezedried. Titers determined after processing indicated that, of the
more » ... h, glycerol, and freeze-dry methods, freezedrying was most damaging to the phages tested, glycerol less damaging, and the broth method least damaging. After 2 years, titers of broth lysates were generally higher than those of glycerol or freeze-dried preparations. Dried preparations generally did not prove satisfactory. Preparations stored at 4 C showed better titers than those kept at room temperature. All titers declined with time regardless of the conditions of preservation. 466 on December 11, 2020 by guest Downloaded from 1962] 471 on December 11, 2020 by guest Downloaded from
doi:10.1128/am.10.5.466-471.1962 fatcat:kk4vgjjh4ja4vajfjm5dipbdka