U Mass - Organ-specific glucose uptake v1 (protocols.io.x4cfqsw) [dataset]

Jason Kim
2019 protocols.io  
Summa ry: Summa ry: Glucose uptake in individual organs can be measured using a bolus injection of 2-deoxy-D-[1-14 C] glucose, a non-metabolizable glucose analog, and by determining labeled metabolite levels in select tissues. Insulin resistance is characterized by reduced glucose metabolism and develops in obese mice. Bio-rad Laboratories Re a ge nt Pre pa ra ti on: Re a ge nt Pre pa ra ti on: Re a ge nt 1 Re a ge nt 1 : 0.2 M formic acid/0.5 M ammonium acetate Reagents and Materials: formic
more » ... Materials: formic acid, ammonium acetate, deionized water Procedure: 1. Prepare 900 ml of dH ² O, and add 7.69 ml of formic acid. 2. Add 38.84 g of ammonium acetate, and adjust pH to 4.9±0.05 using dH ² O. 3. Add dH ² O to make a final solution volume of 1,000 ml. Note : Note : Bi o-Ra d L a bora tori e s Bi o-Ra d L a bora tori e s RRID:SCR_008426 RRID:SCR_008426 Si gma -Al dri ch Si gma -Al dri ch RRID:SCR_008988 RRID:SCR_008988 1 Survival surgery is performed to establish a chronic indwelling catheter at 5~6 days prior to experiment for intravenous infusion. (refer to M1023: Surgery-jugular vein cannulation) 2 Mice are fasted overnight (~15 hours) or for 5 hours prior to the start of experiment. 3 Place a mouse in a rat-size restrainer with its tail tape-tethered at one end.
doi:10.17504/protocols.io.x4cfqsw fatcat:itw7coh53fabvh776tger7sdau