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Summa ry: Summa ry: Glucose uptake in individual organs can be measured using a bolus injection of 2-deoxy-D-[1-14 C] glucose, a non-metabolizable glucose analog, and by determining labeled metabolite levels in select tissues. Insulin resistance is characterized by reduced glucose metabolism and develops in obese mice. Bio-rad Laboratories Re a ge nt Pre pa ra ti on: Re a ge nt Pre pa ra ti on: Re a ge nt 1 Re a ge nt 1 : 0.2 M formic acid/0.5 M ammonium acetate Reagents and Materials: formicdoi:10.17504/protocols.io.x4cfqsw fatcat:itw7coh53fabvh776tger7sdau