Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%−90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation (IVM) was measured by fluorescent quantitative RT-PCR method. BMP15 was expressed at low levels in immature oocytes and increased to the highest level at 16 h of IVM, which coincides with the time of cumulus cell

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... expansion, then declined slowly under IVM cultivation. The expression pattern of BMP15 suggested that it might be important in cumulus expansion in rabbits. 兔骨形态发生蛋白 15 cDNA 部分序列的克隆及其在卵母细胞中的表达 尹 萍,季金强,李 霖,丁家桐 * (扬州大学 动物科学与技术学院,扬州 225009) 摘要:采用 RT-PCR 法从新西兰大白兔卵巢中克隆出 BMP15 基因部分 cDNA 片段,经 blast 分析后,发现其 与猪、 牛、 绵羊、 山羊、 人和小鼠的同源性达到了 83%-90%。 同时, 利用实时荧光定量 RT-PCR 方法检测了 BMP15 mRNA 在卵母细胞体外成熟培养过程中(IVM)的表达变化情况,结果表明:在兔卵母细胞体外成熟过程中,该 基因在未成熟卵母细胞中表达水平较低,在培养 16 h 其表达丰度达到峰值,并与卵丘扩展时间相一致,随后下降, 推测 BMP15 可能在兔卵丘细胞扩展中发挥重要作用。 关键词:兔;骨形态发生蛋白 15;卵母细胞;克隆;荧光定量 RT-PCR 中图分类号: Q344.13; Q785; Q959.837 文献标识码:A