This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the

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... e of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene. K e y w o r d s Milk, Cheese, E. coli, ETEC, PCR.