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The current study was carried out to determine the effect of secondary metabolites on in vitro rumen microbial fermentation and their potential to inhibit methane (CH 4 ) production involved in the global warming and greenhouse-gases (GHG). The obtained results showed that the addition of Yucca schidigera and Quillaja saponaria reduced significantly CH 4 production (P <0.05). This reduction is proportional to the concentration and it becomes superior to 60 % beyond 8 mg / mL of saponins. CH 4doi:10.1016/j.egypro.2015.07.513 fatcat:okfks67ulrexffsie5tqzk6yma