We conducted a laboratory study to evaluate the efficacy of control agents against small larvae, large larvae, and pupae of Aedes aegypti to determine an appropriate larvicide regime to employ in emergency dengue control programs. The control agents included Bacillus thuringiensis var. israelensis (Bti), pyriproxyfen (an insect growth regulator), a larvicidal oil, Aquatain AMF (polydimethylsiloxane, a monomolecular film), and temephos at the recommend application dosages and rates. Our results

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... howed that Bti, pyriproxyfen, and temephos were efficacious (100z mortality) against larvae, irrespective of the instar stage, but not against pupae of Ae. aegypti (1.5-7.8z mortality). Aquatain AMF, on the other hand, was very effective at controlling the pupal stage (100z mortality), but had limited efficacy against small larvae (38.0z mortality) and large larvae (78.0z mortality). The larvicidal oil was effective against all immature stages (93.3-100z mortality). Therefore, we concluded that for effectively interrupting the dengue transmission cycle, larvicides that kill the pupal stage (Aquatain AMF or larvicidal oil) should be included in an emergency dengue control program in addition to Bti, pyriproxyfen, or temephos.