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Role of the Charge Interaction between Arg70and Asp120in the Tn10-encoded Metal-Tetracycline/H+Antiporter ofEscherichia coli
2000
Journal of Biological Chemistry
We reported that the positive charge of Arg 70 is mandatory for tetracycline transport activity of Tn10-encoded metal-tetracycline/H ؉ antiporter (TetA(B)) (Someya, Y., and Yamaguchi, A. (1996) Biochemistry 35, 9385-9391). Arg 70 may function through a charge-pairing with a negatively charged residue in close proximity. Therefore, we mutated Asp 66 and Asp 120 , which are only two negatively charged residues located close to Arg 70 in putative secondary structure of TetA(B) and highly conserved
doi:10.1074/jbc.275.1.210
pmid:10617606
fatcat:646oo5oomnfp5iz5kdd6majuvq