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Escherichia coli is a well-studied anaerobic bacteria which is able to regulate metabolic pathways depending on the type of sugar presented in the medium. We have studied the glucose-lactose shift in E. coli at the protein level using a recently developed mass spectrometry platform. Method: Cells were grown in minimal medium containing two sugars (glucose and lactose) and analyzed using novel mass spectrometry cluster. The cluster combines the high resolving power and dynamic range of Fourierdoi:10.1186/1471-2180-11-126 pmid:21631920 pmcid:PMC3121593 fatcat:ngafrgwzdvbnpfy7sqorca4ij4