Rapid identification ofEscherichia colisafety and laboratory strain lineages based on Multiplex-PCR

Andreas Peter Bauer, Sarah Maria Dieckmann, Wolfgang Ludwig, Karl-Heinz Schleifer
2007 FEMS Microbiology Letters  
Escherichia coli K-12, B, C and W strains are the most frequently used bacterial safety and laboratory strains. Lineage-specific DNA fragments were detected by microplate subtractive hybridization and utilized to create a fast differentiation method using a single PCR reaction to differentiate clearly the four lineages and separate them from pathogenic variants. The method has been evaluated on a comprehensive selection of widely used laboratory strains and a variety of pathogenic E. coli
more » ... entatives. In addition, in silico analysis on all available E. coli genomes and the genomes of the close relatives Shigella and Salmonella confirmed the reliability of the proposed method. A fast identification and differentiation of E. coli safety strains by Multiplex-PCR is a useful tool for researchers and companies to check and monitor their reference stocks.
doi:10.1111/j.1574-6968.2006.00594.x pmid:17343689 fatcat:zgbqygkztffmbiguw3vjrn3mvu