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Floxin, a resource for genetically engineering mouse ESCs
2009
Nature Methods
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations are reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable the high-throughput modification of many genes in mouse ESCs. Genetic modification of mice and of
doi:10.1038/nmeth.1406
pmid:19966808
pmcid:PMC2895430
fatcat:7lqdrr3qxrchlpz4l3wdfuy3ly