EDTA-Dependent Pseudothrombocytopenia in a Child. Possible Immunological Mechanism
EDTA依存性偽性血小板減少症の1小児例 免疫学的発症機序に関する考察

1994 The Japanese Journal of Pediatric Hematology  
The mechanism that attributes to EDTA-dependent pseudothrombocytopenia in a 10-years-old boy was immunologically studied by using flow cytometry. Platelets aggregates were brought by incubating with patient serum in the presence of EDTA and was stained with FITC-conjugated goat anti-human immunoglobulin G (IgG) polyclonal antibodies. Moreover, the potential of mouse monoclonal antibody to react with the determinant specific for glycoprotein Hb/Illa complex on platelets was totally abrogated
more » ... platelets were pre-incubated with the patient serum together with EDTA. These data indicate the presence of EDTA-dependent IgG antibodies in the patient serum which provoke platelet aggregation to react specifically with glycoprotein IIb/ Ma complex on the surface of platelets.
doi:10.11412/jjph1987.8.230 fatcat:zvtysb7bhjdpbg2uzpnbor6xeq