The mechanism that attributes to EDTA-dependent pseudothrombocytopenia in a 10-years-old boy was immunologically studied by using flow cytometry. Platelets aggregates were brought by incubating with patient serum in the presence of EDTA and was stained with FITC-conjugated goat anti-human immunoglobulin G (IgG) polyclonal antibodies. Moreover, the potential of mouse monoclonal antibody to react with the determinant specific for glycoprotein Hb/Illa complex on platelets was totally abrogated

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... platelets were pre-incubated with the patient serum together with EDTA. These data indicate the presence of EDTA-dependent IgG antibodies in the patient serum which provoke platelet aggregation to react specifically with glycoprotein IIb/ Ma complex on the surface of platelets.