Identification of a Firefly Luciferase Active Site Peptide Using a Benzophenone-based Photooxidation Reagent

Bruce R. Branchini, Rachelle A. Magyar, Karen M. Marcantonio, Kate J. Newberry, Justin G. Stroh, Linda K. Hinz, Martha H. Murtiashaw
1997 Journal of Biological Chemistry  
1997) J. Biol. Chem. 272, in press. 2 The abbreviations used are: BPTC, 2-(4-benzoylphenyl)thiazole-4carboxylic acid; HOPhHA, 6-(4-hydroxyphenyl)hexanoic acid; LC/ ESMS, tandem high performance liquid chromatography-electrospray ionization mass spectrometry; NaPB, 0.1 M sodium phosphate buffer (pH 8.0); RP-HPLC, reverse phase-high performance liquid chromatography; TFA, trifluoroacetic acid. HHGF, histidylhistidylglycylphenylalanine; VRGPM, valinylarginylglycylprolylmethionine.
doi:10.1074/jbc.272.31.19359 pmid:9235934 fatcat:erfhdx4g2re2zaezxiiaidgtzy