Control of Contamination in Psittacosis Specimens by Antibiotics

Vester J. Lewis, Helen M. Engelman
1971 Applied microbiology  
Diluent containing a combination of vancomycin, kanamycin, and streptomycin is shown to be more effective than diluent previously used for isolating Clhiamydia psittaci from bird tissue specimens. Attempts to isolate Chiamydia psittaci are frequently thwarted by bacterial contamination. For example, tissue suspensions from 32 of 100 birds suspected of psittacosis that were submitted to the Center for Disease Control from diverse localities during a recent 3-month period yielded only bacteria.
more » ... ese suspensions contained 2 mg of streptomycin per ml, 20 times that suggested for putrifled specimens (1). The present study was undertaken to determine whether adding vancomycin and kanamycin would control contamination more effectively.
doi:10.1128/aem.22.3.473-474.1971 fatcat:rrtb4qi4dffutnpokcyiurxyee