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Diluent containing a combination of vancomycin, kanamycin, and streptomycin is shown to be more effective than diluent previously used for isolating Clhiamydia psittaci from bird tissue specimens. Attempts to isolate Chiamydia psittaci are frequently thwarted by bacterial contamination. For example, tissue suspensions from 32 of 100 birds suspected of psittacosis that were submitted to the Center for Disease Control from diverse localities during a recent 3-month period yielded only bacteria.doi:10.1128/aem.22.3.473-474.1971 fatcat:rrtb4qi4dffutnpokcyiurxyee