Fermentative Production of Exocellular Glucans by Fleshy Fungi

E. N. Davis, R. A. Rhodes, H. Russell Shulke
1965 Applied microbiology  
DAVIS, E. N. (Northern Regional Research Laboratory, Peoria, Ill.), R. A. RHODES, AND H. RUSSELL SHULKE. Fermentative production of exocellular glucans by fleshy fungi. Appl. Microbiol. 13:267-271. 1965.-Two specimens of higher fungi produced exocellular ,8-1,3-glucans when their mycelial forms were cultivated under submerged aerobic conditions. Plectania occidentalis NRRL 3137 consumed up to 6% glucose or xylose with about 30% conversion to polymer in a medium composed of hydrolyzed soy
more » ... , salts, and thiamine. A 5% inoculum was used in a 10-day shaken fermentation. After dilution of the culture liquors and partial disruption of mycelia with a blender, solids were removed by centrifugation, and the polymer was precipitated by the admixture of 2 volumes of ethyl alcohol. A second polymer was formed in 40 to 65% yield by fermentation with Helotium sp. NRRL 3129, which in the imperfect stage would be identified as Monilia sp. It consumed up to 4% glucose, fructose, mannose, or sucrose in 60 to 72 hr. A 2% inoculum in a medium composed of commercial defatted soy flakes, phosphate, and thiamine in tap water gave a satisfactory fermentation. This polymer was precipitated by the addition of 0.5 volume of ethyl alcohol. Both organisms have a broad pH optimum on the slightly acidic side and did best at about 25 C. flasks; these were sterilized at 121 C for 18 min. A piece of mycelium cut from an agar slant (medium 267 on May 7, 2020 by guest
doi:10.1128/aem.13.2.267-271.1965 fatcat:yzn25id5fjasngrm4cc4xrmr7q