Reproducibility warning: The curious case of Polyethylene glycol 6000 and spheroid cell culture [article]

Silke Krol, Simona Serrati, Chiara Martinelli, Antonio Palazzo, Rosa Maria Iacobazzi, Mara Perrone, Roberto Santoliquido, Quy K. Ong, Zhi Luo, Ahmet Bekdemir, Giulia Pinto, Ornella Cavalleri (+4 others)
2019 bioRxiv   pre-print
In this study we report about the reproducibility of three-dimensional cell culture of floating cell spheroids on PEG6000 treated cell culture dishes. Three-dimensional tumour spheroids or organoids present an interesting test platform for nanoparticulated drug delivery or nanoparticle toxicity. We tested the reproducibility of spheroid formation induced by the PEG coated surface. Interestingly we found that the results were different in a reproducible manner depending on the distributors of PEG6000.
doi:10.1101/793828 fatcat:snje44xe3nctpnppik3kqbnpdm