While increasing lipase production by Mucor javanicus IAM 6108 , reduction in cultivation time and stabilization of lipase in the broth were investigated. The addition of 1 % soybean oil to the initial medium containing 3 % corn steep liquor, 1 % soluble starch, 0.2 % KH2PO4, 0.05 % MgSO4.7 H2O, 0.05 % KC1, and 1 % soybeanyuto reduced the cultivation time of lipase production from 50 h to 30 h. Lipase activity in the broth decreased rapidly after reaching a maximum . To avoid this, the 1 %

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... an oil was added to the broth at 25 h of cultivation before the maximum had been reached , As a result, maximum lipase activity was maintained for more than 10 h. This appeared due to the presence of fatty acids or triglycerides with double bonds on the broth.