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Journal of Virology
Mouse bioassay remains the gold standard for determining proof of infectivity, strain type, and infectious titer estimation in prion disease research. The development of an approach using ex vivo cell-based assays remains an attractive alternative, both in order to reduce the use of mice and to hasten results. The main limitation of a cell-based approach is the scarcity of cell lines permissive to infection with natural transmissible spongiform encephalopathy strains. This study combines twodoi:10.1128/jvi.01855-09 pmid:20032176 pmcid:PMC2820909 fatcat:dsrvlk2q2rbszkvqz5c362rfvm