Blood platelets are generated in the bone marrow (BM) from their precursors, megakaryocytes (MK). Although we know that MKs produced many platelets throughout life, precisely how platelets are produced and how circulating numbers are tightly regulated in vivo remain uncertain, largely because of the rarity of MKs in the BM and the lack an adequate visualization technique. Thus, we utilized highspeed XYZT two photon imaging using GaAs detectors, and rapid resonance scanning mirrors for

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... visualization of BM MKs. This system enabled us to evaluate the detailed thrombopoietic processes from BM MKs.