Scanning acoustic microscope (SAM) is a useful observational tool in cellular study as living observation which is non-invasive is feasible compared to conventional optical microscope. In previous study, the cell morphological change by acoustic impedance measurement were successfully visualized. These acoustic impedance changes correspond with cell elasticity mainly reflects the changes of the cytoskeleton. In this study, we evaluate the elastic change of murine breast cancer cell, C127I

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... mitosis. C127I cells were cultured to ~75% confluency before measurement, using a transducer with a central frequency of 320 MHz. Dynamic changes during mitosis were successfully mapped using SAM and confirmed by laser confocal microscopy. Cells in prometaphase, anaphase and telophase which can only be confirmed through immunostaining before were successfully visualized using SAM. This suggests that SAM is capable to distinguish cell in different mitotic phase based on the changes of acoustic impedance.