ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli

F. Agostini, D. Cirillo, C. M. Livi, R. Delli Ponti, G. G. Tartaglia
2014 Bioinformatics  
Here we introduce ccSOL omics, a webserver for largescale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis. Results: Using coil/disorder, hydrophobicity, hydrophilicity, -sheet and -helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36 990 Target Track entries). Validation on
more » ... ee independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31 760 proteins sharing 530% sequence similarity. Availability and implementation: ccSOL omics can be freely accessed on the web at
doi:10.1093/bioinformatics/btu420 pmid:24990610 pmcid:PMC4184263 fatcat:yxsvnphkdvadpgix5w5p3la5me