Abstracts from the 2016 Joint Meeting of the International Confocal Group (ICG), the International Dermoscopy Society (IDS), and the International Society for Digital Imaging of the Skin (ISDIS)

2016 Dermatology Practical & Conceptual  
Spectrally encoded confocal microscopy (SECM) is a highspeed reflectance confocal microscopy technology. SECM utilizes a stationary optical element, a diffraction grating, and a broadband light source to illuminate a line on the tissue with multiple spectrally-encoded spots, which enables scan-less line confocal imaging. In order to conduct two-dimensional confocal imaging, however, SECM still needs to use a beam scanning device or needs to mechanically translate the device. When a slit
more » ... is used, SECM can image a rectangular area of the tissue with multiple spectrally-encoded lines, which enables two-dimensional confocal imaging without using any beam scanning devices. Resulting confocal images are directly projected on a two-dimensional imaging sensor. This new approach, slit-SECM, can uniquely enable development of a smartphone confocal microscope, in which an optics module is attached to a smartphone to conduct confocal imaging and the smartphone imaging sensor is used to acquire two-dimensional confocal images. Due to its low cost, portability, and inherent network connectivity, smartphone confocal microscopy has a potential to provide an in vivo diagnostic tool in resource-poor countries and also to increase clinical adaptation of confocal imaging in developed countries. We present preliminary results of imaging human skin in vivo with slit-SECM. We developed a slit-SECM bench system with an inexpensive, battery-powered LED ($25) and a low-cost color CMOS sensor ($355). The bench system achieved lateral resolution of 1.3 µm and axial resolution of 6 µm. Confocal images of human skin were acquired at the speed of 10 frames/sec. Acquired confocal images clearly visualized characteristic cellular features of human skin down to the dermal-epidermal junction, including cell nuclei in spinous layer and dermal papilla. Results from this preliminary study show feasibility of conducting skin confocal imaging using inexpensive optical and electrical components and suggest that slit-SECM may be developed into a low-cost smartphone confocal microscope.
doi:10.5826/dpc.0602a11 fatcat:3kt5cdcquvhj3awhnh7rutowp4