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Insights into the mutational burden of human induced pluripotent stem cells using an integrative omics approach
To understand the mutational burden of human induced pluripotent stem cells (iPSCs), we whole genome sequenced 18 fibroblast-derived iPSC lines and identified different classes of somatic mutations based on structure, origin and frequency. Copy number alterations affected 295 kb in each sample and strongly impacted gene expression. UV-damage mutations were present in ~45% of the iPSCs and accounted for most of the observed heterogeneity in mutation rates across lines. Subclonal mutations (notdoi:10.1101/334870 fatcat:s77ptz3crbgxtfnyxuwxkyfmhi