12518.8 滋g·L -1 相差不显著, 响应面法能较好地优化产朊假丝酵母富硒工艺条件。 关键词: 产朊假丝酵母; 富硒; 响应面法; 总硒含量 中图分类号: TS201.3 文献标志码: A 文章编号: 2095-6819 (2014) 06-0575-08 Abstract: Thefermentationconditionsofseleniumenrichmentby Candidautilis werestudied.Basedontheresultsofthesinglefactorexperi原 ment,threefactorsincludingtheconcentrationofsodiumselenite,initalpHandincubationtemperaturewereselected.Theresponsesurface methodwasusedtooptimizethevariousfactors.Theoptimalconditionswereobtainedasfollows:incubationtimewas30h,timeofadding

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... eleniumwasmid-logarithmic,thesodiumseleniteconcentrationwas35mg·L -1 withinitalpHof6.6,incubationconcentrationof10%,incu原 bationtemperatureof27 益,themediumvolumeof150mL/500mL,respectively.Undertheoptimalcondition,thebiomasswas6.87g · L -1 . Thetotalseleniumcontentof Candidautilis was12639.7 滋g·L -1 ,andtheseleniumcontentofthecellswas1839.8 滋g·g -1 ,inwhichsodium seleniteconversionratewas79.1%andtheorganicseleniumwashigherthan90%.Theactualvalueofseleniumcontentwassubstantially consistentwiththetheoreticalvalue,andtheresponsesurfacemethodologywasapplicableforthefermentationconditionsofseleniumen原 richedby Candidautilis.