and Background: Cyperus rotundus Trigonella foenum graecum (fenugreek) have been utilized for many years as medical applications, mostly as traditional natural medications. The present research was designed to assess the hypoglycemic activity of the ethanol extract of Iraqi fenugreek seeds and tuberous roots of in alloxan-stimulated C. rotundus diabetic rats and to evaluate the diverse phytochemicals found in ethanol extracts that may have therapeutic activity. Qualitative assessment of diverse

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... secondary metabolites by Methods: corresponding chemical tests was been used to specify each group of compounds in the crude extracts of both plants. Male Wistar rats (n=40) were subdivided randomly into four main groups: group A, alloxan-stimulated diabetic rats with administered with distilled water (10mL/kg; control); group B-D, alloxan-stimulated diabetic rats administered with ethanol extract of (10 mg/kg; group B), C. rotundus glibenclamide (10 mg/kg; group C), and ethanol extract of fenugreek seeds (15mg/kg; group D). These medications were given orally for 6 weeks. Blood glucose of the rats was measured at baseline and after 1, 2, 4, and 6 weeks after administration of medication. Phytochemical analysis showed the presence of many secondary Results: metabolites including alkaloids, flavonoids, anthraquinones, tannins, and steroids, in the crude extracts of and fenugreek seeds. After 6 C. rotundus weeks all groups of rats showed a significant decline in blood sugar level compared to the control group. Group C (glibenclamide) showed the lowest value, followed by group D (fenugreek seeds) and group B (C. rotundus). Ethanol extract of both plants possess considerable Conclusions: hypoglycemic activity in alloxan-stimulated diabetic rats, with superior efficacy shown by fenugreek seed extract, after 6 weeks of study . Khamees AH, Fawzi HA and Sahib HB. How to cite this article: Phytochemical investigation and assessment of the hypoglycemic activity of two herbal extracts from selected Iraqi medicinal plants in alloxan-stimulated diabetic rats: a comparative study [version 1; peer F1000Research 2020, :247 review: 1 approved with reservations] 9 https://doi.