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PURPOSE. To reexamine whether the ␣5 chain of type IV (␣5 [IV]) collagen, thought to be absent, is in fact present in human amniotic membrane. METHODS. Cryosections of human amniotic membrane obtained at Cesarean section were immunohistochemically examined for the presence of ␣5(IV), with or without inclusion of the denaturing step. Amniotic membrane was digested with collagenase to release the noncollagenous NC1 domain from the ␣-chain. The NC1 domain of ␣5(IV) was then assayed on Western blotdoi:10.1167/iovs.03-0952 pmid:15161839 fatcat:zv6gdep755a3jgz3xgf5sxywqy