Non fermentative gram negative bacilli that were considered to be contaminants in the past have now emerged as important health care associated pathogens. Aim: This study was undertaken to identify and characterize Nonfermenters isolated from various clinical samples. Material and Methods: Present study was conducted in the department of Microbiology, National Institute of Medical Sciences (NIMS) Medical College and hospital, Jaipur from July 2013 to July 2014. A total 150 strains of

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... rs were isolated from 1200 various non repetitive clinical samples. The samples were processed and Non fermentative gram negative bacilli (NFGNB) identified as far as possible up to species level as per standard protocols. Results: In the present study, 12.50% of incidence / isolation rate of Nonfermenters in various clinical samples was found. Out of 150 strains of Nonfermenter, Pseudomonas aeruginosa was the most common isolate 134 (89.33%) followed by Acinetobacter baumannii 16 (10.67%). 89 (59.33%) NFGNB were isolated from males and 61 (40.67%) were from females. They were mainly isolated from age group 21-30 Years. Higher rate of isolation of NFGNB were 85 (56.67%) from IPD and 38 (25.33%) from ICU patients. Among all clinical samples Pus and Wound Discharge yield maximum isolates of NFGNB i.e. 54 (36%) followed by Sputum 39 (26.0%). Conclusion: NFGNB should not be ignored as mere contaminant but correlate clinically for its pathogenic potential and identified using standard protocol so as to institute appropriate and timely antibiotic coverage Keywords: Identification, Nonfermenter, Pseudomonas, Acinetobacter