Stem cell like Periodontal Ligament Stem Cells (PDLSCs) and Stem Cells derived from Exfoliated Deciduous Teeth (SHEDs) suggested to be reliable stem cell source for clinical utility to repair damaged tooth tissue. However, the expansion of stem cells using animal serum (FBS) have several disadvantages. Though human platelet lysate (PL) found to be an alternative substitute for FBS, optimum concentration and its biological effect on PDLSCs 0and SHEDs remains to be explored. PDLSCs and SHEDs were

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... isolated from dental tissues and stem cell marker characterization carried out by flow cytometry. Expression of osteogenic and neurogenic differentiation markers evaluated by quantitative Real Time PCR (qRT-PCR). Mineralized potential of PDLSCs and SHEDs was assessed by Alizarin Red Staining method. PDLSCs and SHEDs proliferation was significantly elevated when cells treated with 5% PL compared to 10% FBS. Moreover, both cells showed enhanced mineralization ability and neurogenic differentiation at 5% PL. Moreover, SHEDs expressed increased neurogenic differentiation potential compared to PDLSCs at 5% PL concentration. Our results suggested that at 5% optimum PL concentration both PDLSCs and SHEDs shows increased osteogenic and neurogenic differentiation ability compared to FBS.