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ISOLATION AND CHARACTERIZATION OF SOLUBLE POLYSACCHARIDES FROM CALAMAGROSTIS ANGUSTIFOLIA KOM
2011
BioResources
Sequential treatments of dewaxed Calamagrostis angustifolia Kom with water (60 ºC and 90 ºC), 70% ethanol, and 70% ethanol containing 0.2%, 1.0%, 2.0%, 4.0%, and 8.0% NaOH at a solid to liquid ratio of 1:25 (g/mL) at 80 ºC for 3 h yielded 36.2% soluble polysaccharides of the dry dewaxed material. The eight polysaccharide fractions obtained were comparatively studied by sugar analysis, GPC, FT-IR, 1H and 13C-NMR, and 2D-NMR (HSQC) spectroscopy. The results showed that the water-soluble
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