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This document presents the standardized protocol for placing landmarks on virtual 3D renderings of subadult crania using the Amira visualization software. This protocol was developed as part of National Institute of Justice Award 2019 DU-BX-0039. The new version (v2) has been updated to include corrected figures.doi:10.5281/zenodo.6625998 fatcat:dhivdzdezrbylfogx2mzifgytm