Original Article High ω-3/ω-6 ratio diet could alleviate blood-brain barrier dysfunction in rat model with traumatic brain injury

Jianguang Su, Kai Quan, Kun Fan, Jiaying Zhang, Jingfang Liu, Wei Hua, Ying Mao
2016 Int J Clin Exp Med   unpublished
Objectives: Polyunsaturated fatty acids (PUFAs) have functional activity in many aspects, including brain trauma. Diets with different ratios of ω-3/ω-6 were applied to investigate the protective effect on delayed blood brain barrier (BBB) dysfunction after traumatic brain injury (TBI) in rat models. Methods: Moderate TBI was mimicked by Feeney's weight-dropping rat models. The effects of PUFA, with different ω-3/ω-6 ratios (1:1, 1:5, and 1:30) on BBB disruption, were examined by Evans blue
more » ... d by Evans blue (EB) dye varying from different time (day 3, 5, 7) after TBI. The corresponding change of 4-HNE and tight junction proteins were analyzed by immunofluorescence staining and western blotting technology. In the meantime, D-penicillamine (4-HNE inhibitor) was administrated to verify the role of 4-HNE in this event. Cognitive function was evaluated by Morris water maze test. Results: Delayed BBB disruption was observed in the injured brains. High ω-3/ω-6 ratios diet significantly reduced EB extravagation and 4-HNE level, and also enhanced the expression of tight junction proteins (ZO-1 and occludin). Inhibiting 4-HNE by administrating D-penicillamine could exert protective effects as well. In addition, high ω-3/ω-6 ratios diet could also promote the recovery of cognitive function after TBI. Conclusions: High ω-3/ω-6 ratio diet could alleviate delayed BBB dysfunc-tion, which was associated with reduction of 4-HNE and enhancement of tight junction proteins. Besides, 1:1 might be a more suitable dietary ω-3/ω-6 ratio for TBI patient.