EVALUATION OF "LEUCAS LANATA" AND ASSESSMENT OF ITS HEPATOPROTECTIVE EFFECTS ON ANTITUBERCULAR DRUG-INDUCED HEPATIC DAMAGE

Pritt Verma, Sajal Srivastava, Ch. V. Rao
2018 Asian Journal of Pharmaceutical and Clinical Research  
Objective: The objective of this study was to investigate the hepatoprotective activity of ethanolic whole plant extract of Leucas lanata (family - Lamiaceae) against rifampicin+isoniazid (RIF+INH) induced hepatic damage in rats.Methods: Wistar rats of either sex were divided into five groups of six animals each and given orally the following treatment for 28 days. The normal control was given 1% CMC 1 ml/kg body weight (b.w.), RIF+INH antitubercular drug (ATT) at a dose of 50 mg/kg b.w., p.o.
more » ... 0 mg/kg b.w., p.o. was given a toxic dose for inducing hepatotoxicity. Silymarin (100 mg/kg, p.o) was given as reference standard. Two different doses of Lucas lanata extract of 200 and 400 mg/kg, p.o were tested for hepatoprotective activity. At the end of the treatment, blood was collected from direct cardiac puncture and analyzed for numerous bodily fluid parameter such as alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), total protein (TP), and total bilirubin (TB) and oxidative stress parameter lipid peroxidation (LPO), superoxide dismutase (SOD), catalase, glutathione (GSH), malondialdehyde and also evaluated the hydroxyproline content, tumor necrosis factor alpha, and interleukin-1 beta in all groups. Livers were isolated for its essential estimation followed by histopathological studies.Results: Hepatic serum markers (ALT, AST, ALP, albumins, TP, and TB) were significant (p<0.01–p<0.001) protective effect was obtained against ATT-induced liver damage, and ATT treatment significantly decreased LPO, SOD, and GSH (p<0.001) levels compared with control Group I and given a Leucas lanata extract (LLE) (p<0.01–p<0.001), the oxidative stress markers were markedly reversed. Histopathology of liver tissue showed that LLE attenuated the hepatocellular necrosis, regeneration, and repair of cells toward normal. LLE which was standardized using high-performance thin-layer chromatography revealed the presence of some critical phenolic (gallic acid and quercetin) compound.Conclusion: The results of this study powerfully indicate the protecting result of LLE against liver injury which can be attributed to its hepatoprotective activity, and thereby scientifically support its traditional use.
doi:10.22159/ajpcr.2018.v11i6.25888 fatcat:cz2dkvvegvcyjb6xe2t2cdobna