Methanolic Extracts of Solieria robusta Inhibits Proliferation of Oral Cancer Ca9-22 Cells via Apoptosis and Oxidative Stress

Yii-Huei Yen, Ammad Farooqi, Kun-Tzu Li, Ghazala Butt, Jen-Yang Tang, Chang-Yi Wu, Yuan-Bin Cheng, Ming-Feng Hou, Hsueh-Wei Chang
2014 Molecules  
Many red algae-derived natural products are known to have anticancer effects. The biological functions of the red alga Solieria robusta from the Karachi coast (Pakistan) remain unclear. Here, we prepared a methanolic extracts of S. robusta (MESR) to examine its possible anti-oral cancer effects and the corresponding mechanism of action. Cell viability of MESR-incubated oral cancer Ca9-22 cells was dose-responsively decreased (p < 0.001). According to a propidium iodide (PI)-based assay the cell
more » ... cycle distribution was dramatically changed, especially for subG1 accumulation. Annexin V/PI assay of apoptosis using flow cytometry also showed that MESR-incubated Ca9-22 cells were dose-responsively increased (p < 0.001). For evaluation of oxidative stress in MESR-incubated Ca9-22 cells, we found that reactive oxygen species (ROS) were overexpressed dose-and time-responsively and mitochondrial depolarization was also increased (p < 0.001). Taken together, MESR showed inhibitory effects on oral cancer proliferation coupled with apoptosis and oxidative stress.
doi:10.3390/molecules191118721 pmid:25405289 fatcat:ih233wujgfdgpcmjliri4eq5cu