PRECLINICAL STUDY OF THE DNA-DAMAGING ACTIVITY AND GENOTOXICITY OF CARBAMYLATED DARBEPOETIN

Pavel Kolesnichenko, Sergey Nadezhdin, Yuri Burda, Elena Artyushkova, Natalya Bystrova, Galina Lazareva, Vladimir Provotorov, Pavel Kolesnichenko
IAJPS   unpublished
The article presents the results of a potential mutagenicity studying of a new drug from the group of erythropoietin, carbamylateddarbepoetin. The study was performed using DNA-comet assay in mice, and in micronucleus test. As a positive control there were used mice which had been injected with genotoxicantmethylmethane sulfonate at a dose of 40 mg/kg and as a negative control there were mice after administration of equivalent doses of a placebo. The results showed that the mutagenic effect of
more » ... he drug in single (3.59±1.02) dosing and three times (4.0±0.78) dosing had not been different from the mutagenic effect of placebo (4.39±1.34). Micronucleus test was performed using a cytogenetic damage of bone marrow cells and checking the appearance of polychromatophil cells, containing micronuclei. It was established that carbamylateddarbepoetin within stated doses according to the used test, has no a potential carcinogenic effect.
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