STIM1 Knockdown Reveals That Store-operated Ca2+Channels Located Close to Sarco/Endoplasmic Ca2+ATPases (SERCA) Pumps Silently Refill the Endoplasmic Reticulum

Hélène Jousset, Maud Frieden, Nicolas Demaurex
2007 Journal of Biological Chemistry  
2 The abbreviations used are: ER, endoplasmic reticulum; [Ca 2ϩ ] ER , endoplasmic reticulum free Ca 2ϩ concentration; [Ca 2ϩ ] mit , mitochondrial matrix free Ca 2ϩ concentration; SERCA, sarco/ER Ca 2ϩ -ATPase; STIM1, stromal interaction molecule 1; YC, yellow cameleon; PM, plasma membrane; dsRNA, double-stranded ribonucleic acid; SOC, store-operated Ca 2ϩ ; CRAC, calcium release-activated calcium; siRNA, small interfering RNA; RT, reverse transcription; TAMRA, tetramethylrhodamine; FAM,
more » ... odamine; FAM, 6-carboxyfluorescein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
doi:10.1074/jbc.m609551200 pmid:17283081 fatcat:gwbgo5plwzdmjeg4avrmgz5jfa