Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques

Nawal Thnoon Youis, Qusi Al Jalabi, Thikra Ali
2009 Journal of education and science  
The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G -75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml. The apparent molecular weight of leptin had been determined by gel filtration chromatography technique using Sephadex G -75, and by SDS electrophoresis, the results showed that the apparent molecular weight for the band number 5 of leptin hormone was
more » ... ptin hormone was 15750353 Dalton and 16000 Dalton respectively. * ‫مف‬ ‫مستؿ‬ ‫بحث‬ ‫أطر‬ ‫كحة‬ ‫يكنس‬ ‫ذنكف‬ ‫اؿ‬ ‫نك‬ ‫لمباحثة‬ ‫اه‬ ‫الدكتكر‬ . ‫الحياتية‬ ‫التقنيات‬ ‫باستخدام‬ ‫الجزيئي‬ ‫وزنه‬ ‫وتقدير‬ ‫البالزما‬ ‫من‬ ‫المبتين‬ ‫هرمون‬ ‫عزل‬ .
doi:10.33899/edusj.2009.57654 fatcat:brssclwjjzch5m24ji7lzxdu7m